Journal: Journal of Experimental Botany
Article Title: Laser ablation tomography for visualization of root colonization by edaphic organisms
doi: 10.1093/jxb/erz271
Figure Lengend Snippet: Schematic of the LAT system. The pulsed UV laser source (Coherent Avia 355-7000 Q-switched Nd:YVO4) is directed into beam-shaping optics (HurryScan 10 galvanometer) to transform the beam into an oscillating beam over a linear distance, creating a cutting sheet. The root sample is moved into the beam path on a motorized linear stage (Zaber low vacuum motorized linear stage) as a camera (Canon T3i 18MP camera with a Canon 65 mm MP-E 1–5× variable magnification macro lens) simultaneously images the exposed anatomy ablated by the beam. Reproduced from . Three-dimensional analysis of biological systems via a novel laser ablation technique. Journal of Laser Applications 31, 1–5, with permission from the Laser Institute of America.
Article Snippet: A Canon (Melville, NY, USA) T3i 18MP camera with a Canon 65 mm MP-E 1–5× variable magnification macro lens was used to image each subsequent laser-illuminated slice as the root sample was pushed into the beam path by the stage.
Techniques: